Development of a New Assay for Complex I of the Respiratory Chain

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Development of a new assay for complex I of the respiratory chain.

BACKGROUND Measurement of complex I activity has been hampered by the large amounts of tissue required and the resulting turbidity of the assay solution, which makes spectrophotometric analysis difficult. We have developed a new assay for measuring the activity of complex I in isolated mitochondria that is also applicable to skeletal muscle homogenate in patients with suspected mitochondrial di...

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ژورنال

عنوان ژورنال: Clinical Chemistry

سال: 2000

ISSN: 0009-9147,1530-8561

DOI: 10.1093/clinchem/46.3.345